He was the love interest of Elizabeth Shaw and an archaeologist who was assigned to assist the USCSS Prometheus expedition in discovering the homeworld of the Engineers on LV-223.
Together with her scientific partner and lover, Elizabeth Shaw, Charlie discovered the location of LV-223 and convinced Weyland Corp to fund a scientific expedition to the planet. A skeptic and adventurous archeologist, Holloway's lover and colleague Elizabeth Shaw showed the most religious zeal out of the Prometheus' crew, but he remained always the more objective of the two.
Over the course of the film Holloway is infected with the Engineer´s black liquid by David-8. The infection at first very slowly mutates him and no symptoms are present. Holloway eventually has sex with Shaw and unknowingly impregnates her with an alien monster. After sleeping with Shaw he begins to feel fever and also notices a very small organism in one of his eyes. Later, the infection spreads more and caused him an even more severe feverish state and intense pain afterwards. Other symptoms then start to show, like bloodshot eyes, very visible dark veins, rapidly deteriorating skin tissue and a very pale, almost livid skin color.
To save the life of his beloved Elizabeth and to stop his pain, Holloway is burned alive at his own request.